| Console: | Nintendo Game Boy |
| TV Standard: | Region Not Set |
| Developer(s): | Konami |
| Publisher(s): | Konami |
| Release Date: | 1994-01-01 |
| Players: | 1 |
| Co-op: | No |
| Type: | Shooter |
The Twin Bees, who were living peacefully in Donburi Island, receive a challenge from an unknown individual calling himself Dr. Nikki. "How dare you destroy my creation King Spice, as well as Gattlantis and Poco Poco! This time I'll be your opponent. I'll be waiting for you at Puka Puka Island."
Dr Nikki was none other than Dr. Cinnamon's childhood rival. Whereas Cinnamon decided to use his intellect for the greater good, Nikki became a mad scientist. After reading the challenge, TwinBee and WinBee decide to head to Puca Puca Island without Dr. Cinnamon's knowledge, leaving GwinBee in charge of protecting the scientist. Can the TwinBees truly defeat Dr. Nikki?
Game Player of Pop'n TwinBee on Nintendo Game BoyGenerally, you can review the controls (keyboard) setting during each game in the screen before it starts. Use the arrow keys and “Z”, “X”, “A”, “S”, “Q”, “E” buttons to play, or customize yourself with your gamepad or keyboard.
| Keyboard | Controls |
| ← → ↑ ↓ | Directions for LEFT, RIGHT, UP, DOWN |
| Enter ↵ | START |
| Shift | SELECT |
| Z | A |
| X | B |
| A | X |
| S | Y |
| Q | L |
| E | R |
